The music icon revealed the news ahead of Sunday's headline performance.
The music festival will honour the late greats with some special performances this year.
Glastonbury music festival will honour the late music greats, David Bowie and Prince this year.
The Pyramid Stage will be decorated with a giant lightning bolt, inspired by the artwork for Bowie's 'Aladdin Sane' album.
The Park Stage will also host a performance of Philip Glass' Symphony No.4, which is based on Bowie's 'Heroes' LP.
Festival organiser Emily Eavis said that there are also plans to pay tribute to Prince, who had long been rumoured to headline the festival.
Bowie headlined the festival in 2000 playing most of his hits including 'Let's Dance', 'Heroes' and 'Starman'.
Eavis called the performance "the best festival set I've ever seen". Footage from that performance will also be screened around the festival site this year.
Adele, Coldplay and Muse will headline the Pyramid Stage this year and Eavis says the performances will be "very contrasting but amazing experiences."
Glastonbury Festival will take place at Worthy Farm, Somerset from the 22nd to the 26th of June.