A new video has been released in celebration of the singer's 70th birthday AND the 50th anniversary of his songwriting partnership with Bernie Taupin.
The singer was granted permission to adopt the girls from Malawi just last week.
Madonna shared a heart-warming video of her newly adopted twin daughters, Esther and Stella singing 'Twinkle Twinkle' on Instagram, at the weekend.
The four-year-old girls, dressed in matching blue and white striped pyjamas, are accompanied by Madonna's 11-year-old daughter Mercy on the piano.
The 58-year-old captioned the candid footage, "A little night music" and it has since been viewed more than half a million times.
The singer was officially granted permission to adopt the girls from their home country of Malawi last week.