Join us this Bank Holiday weekend as we play the nation's favourite songs.
The singer was granted permission to adopt the girls from Malawi just last week.
Madonna shared a heart-warming video of her newly adopted twin daughters, Esther and Stella singing 'Twinkle Twinkle' on Instagram, at the weekend.
The four-year-old girls, dressed in matching blue and white striped pyjamas, are accompanied by Madonna's 11-year-old daughter Mercy on the piano.
The 58-year-old captioned the candid footage, "A little night music" and it has since been viewed more than half a million times.
The singer was officially granted permission to adopt the girls from their home country of Malawi last week.