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20 February 2017, 10:04 | Updated: 2 November 2017, 15:29
The singer was granted permission to adopt the girls from Malawi just last week.
Madonna shared a heart-warming video of her newly adopted twin daughters, Esther and Stella singing 'Twinkle Twinkle' on Instagram, at the weekend.
The four-year-old girls, dressed in matching blue and white striped pyjamas, are accompanied by Madonna's 11-year-old daughter Mercy on the piano.
The 58-year-old captioned the candid footage, "A little night music" and it has since been viewed more than half a million times.
The singer was officially granted permission to adopt the girls from their home country of Malawi last week.